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NXF1 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with NXF1 monoclonal antibody, unconjugated (bsm-61943R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with NXF1 monoclonal antibody, unconjugated (bsm-61943R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NXF1 antibody (bsm-61943R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NXF1 antibody (bsm-61943R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • FCM
  • IP

Reactivity

  • Human
Overview
Catalog # bsm-61943r-100uL
Product Name NXF1 Recombinant Antibody
Applications
WB FCM IP
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NXF1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 10482
Swiss Prot Q9UBU9
Subcellular location Nucleus
Synonyms DmNXF1; MEX67; Mex67p; Mvb1; NXF1; Sbr; TAP.
Background Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm.The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IP IP1:20-50

Frequently asked questions

Which applications is NXF1 Recombinant Antibody (bsm-61943R) validated for, and at what dilution?

bsm-61943R is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NXF1 Recombinant Antibody (bsm-61943R) react with?

Tested reactivity: Human.

How should bsm-61943R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise NXF1 Recombinant Antibody (bsm-61943R)?

KLH conjugated synthetic peptide derived from human NXF1

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