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Lysyl tRNA synthetase Recombinant Antibody, AbBy Fluor-405 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61951r-bf405-100uL
Product Name Lysyl tRNA synthetase Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Lysine tRNA ligase
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3735
Swiss Prot Q15046
Subcellular location Extracellular, Cytoplasm, Nucleus, Secreted, Cell membrane
Synonyms CMTRIB; KARS; KARS1; KARS2; KRS; Lysine tRNA ligase; LysRS.
Background Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is Lysyl tRNA synthetase Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61951R-BF405) validated for, and at what dilution?

bsm-61951R-BF405 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Lysyl tRNA synthetase Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61951R-BF405) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61951R-BF405 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Lysyl tRNA synthetase Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61951R-BF405)?

KLH conjugated synthetic peptide derived from human Lysine tRNA ligase

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-61951R-BF405)?

AbBy Fluor-405 on bsm-61951R-BF405: excitation / emission 401nm/421nm.