SAE1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with SAE1 monoclonal antibody, unconjugated (bsm-61972R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with SAE1 monoclonal antibody, unconjugated (bsm-61972R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IP
Flow-Cyt
ICC/IF

Reactivity

Human
Rat

Overview
Catalog #	bsm-61972r
Product Name	SAE1 Recombinant Antibody
Applications	WB, IP, Flow-Cyt, ICC/IF
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SAE1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10055
Swiss Prot	Q9UBE0
Subcellular location	Nucleus
Synonyms	AOS1; HSPC140; Sae1; SUA1; UBLE1A.
Background	The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Application Dilution
WB	1:300-5000
IP	1-2ug
Flow-Cyt	1ug/Test
ICC/IF	1:50-100