SAE1 Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61972r-bf680-100uL |
| Product Name | SAE1 Recombinant Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SAE1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10055 |
| Swiss Prot | Q9UBE0 |
| Subcellular location | Nucleus |
| Synonyms | AOS1; HSPC140; Sae1; SUA1; UBLE1A. |
| Background | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is SAE1 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61972R-BF680) validated for, and at what dilution?
bsm-61972R-BF680 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SAE1 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61972R-BF680) react with?
Tested reactivity: Human, Rat.
How should bsm-61972R-BF680 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SAE1 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61972R-BF680)?
KLH conjugated synthetic peptide derived from human SAE1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-61972R-BF680)?
AbBy Fluor-680 on bsm-61972R-BF680: excitation / emission 679nm/702nm.