SAE1 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61972r-cy3-100uL |
| Product Name | SAE1 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SAE1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10055 |
| Swiss Prot | Q9UBE0 |
| Subcellular location | Nucleus |
| Synonyms | AOS1; HSPC140; Sae1; SUA1; UBLE1A. |
| Background | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is SAE1 Recombinant Antibody, Cy3 Conjugated (bsm-61972R-Cy3) validated for, and at what dilution?
bsm-61972R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SAE1 Recombinant Antibody, Cy3 Conjugated (bsm-61972R-Cy3) react with?
Tested reactivity: Human, Rat.
How should bsm-61972R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SAE1 Recombinant Antibody, Cy3 Conjugated (bsm-61972R-Cy3)?
KLH conjugated synthetic peptide derived from human SAE1
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61972R-Cy3)?
Cy3 on bsm-61972R-Cy3: excitation / emission 512,550nm/570,615nm.