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CBFb Recombinant Antibody, AbBy Fluor-488 Conjugated

Applications

  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61976r-bf488-100uL
Product Name CBFb Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-488
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CBF beta
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 865
Swiss Prot Q13951
Subcellular location Nucleus
Synonyms CBFB; CBFbeta; PEA2; PEA2 beta; PEA2beta; PEBP2 beta; PEBP2B.
Background Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX.
Application Dilution
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is CBFb Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61976R-BF488) validated for, and at what dilution?

bsm-61976R-BF488 is validated for IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CBFb Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61976R-BF488) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61976R-BF488 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CBFb Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61976R-BF488)?

KLH conjugated synthetic peptide derived from human CBF beta

What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-61976R-BF488)?

AbBy Fluor-488 on bsm-61976R-BF488: excitation / emission 499nm/519nm.