HMGA1 Recombinant Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61985r-bf350 |
| Product Name | HMGA1 Recombinant Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HMGA1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3159 |
| Swiss Prot | P17096 |
| Subcellular location | Nucleus |
| Synonyms | HMG R; HMG-I(Y); HMGA1; HMGA1A; HMGIY. |
| Background | HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is HMGA1 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61985R-BF350) validated for, and at what dilution?
bsm-61985R-BF350 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HMGA1 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61985R-BF350) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61985R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HMGA1 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61985R-BF350)?
KLH conjugated synthetic peptide derived from human HMGA1
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bsm-61985R-BF350)?
AbBy Fluor-350 on bsm-61985R-BF350: excitation / emission 346nm/442nm.