Human IgG2 Recombinant Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61986r-bf350-100uL |
| Product Name | Human IgG2 Recombinant Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Human IgG2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P01859 |
| Synonyms | IGHG2; immunoglobulin Gm2; immunoglobulin heavy constant gamma 2 (G2m marker). |
| Background | Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:500-2000 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is Human IgG2 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61986R-BF350) validated for, and at what dilution?
bsm-61986R-BF350 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:500-2000); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Human IgG2 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61986R-BF350) react with?
Tested reactivity: Human.
How should bsm-61986R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Human IgG2 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61986R-BF350)?
KLH conjugated synthetic peptide derived from human Human IgG2
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bsm-61986R-BF350)?
AbBy Fluor-350 on bsm-61986R-BF350: excitation / emission 346nm/442nm.