eIF2A Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with eIF2A monoclonal antibody, unconjugated (bsm-61990R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with eIF2A monoclonal antibody, unconjugated (bsm-61990R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61990r
Product Name	eIF2A Recombinant Antibody
Applications	WB, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human eIF2A
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	83939
Swiss Prot	Q9BY44
Synonyms	65 kDa eukaryotic translation initiation factor 2; Eukaryotic translation initiation factor 2A; ACDA02; EIF 2A; CDA02; MSTP004; MSTP089; EIF2; eif2a; eIF-2A.
Background	Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.
Application Dilution
WB	1:300-5000
ICC/IF	1:50-100