eIF2A Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61990r-cy7 |
| Product Name | eIF2A Recombinant Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human eIF2A |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 83939 |
| Swiss Prot | Q9BY44 |
| Synonyms | 65 kDa eukaryotic translation initiation factor 2; Eukaryotic translation initiation factor 2A; ACDA02; EIF 2A; CDA02; MSTP004; MSTP089; EIF2; eif2a; eIF-2A. |
| Background | Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is eIF2A Recombinant Antibody, Cy7 Conjugated (bsm-61990R-Cy7) validated for, and at what dilution?
bsm-61990R-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eIF2A Recombinant Antibody, Cy7 Conjugated (bsm-61990R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61990R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eIF2A Recombinant Antibody, Cy7 Conjugated (bsm-61990R-Cy7)?
KLH conjugated synthetic peptide derived from human eIF2A
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-61990R-Cy7)?
Cy7 on bsm-61990R-Cy7: excitation / emission 743nm/767nm.