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SIRP Alpha Recombinant Antibody, Cy7 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61992r-cy7
Product Name SIRP Alpha Recombinant Antibody, Cy7 Conjugated
Applications
WB FCM
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SHP substrate 1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 140885
Swiss Prot P78324
Subcellular location Cell membrane
Synonyms CD172a; MFR; MYD 1; p84; PTPNS1; SHPS1; SIRP; SIRPA; SIRPalpha; SIRPalpha1; SIRPalpha2; SIRPalpha3.
Background Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is SIRP Alpha Recombinant Antibody, Cy7 Conjugated (bsm-61992R-Cy7) validated for, and at what dilution?

bsm-61992R-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SIRP Alpha Recombinant Antibody, Cy7 Conjugated (bsm-61992R-Cy7) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61992R-Cy7 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SIRP Alpha Recombinant Antibody, Cy7 Conjugated (bsm-61992R-Cy7)?

KLH conjugated synthetic peptide derived from human SHP substrate 1

What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-61992R-Cy7)?

Cy7 on bsm-61992R-Cy7: excitation / emission 743nm/767nm.