Brachyury Recombinant Monoclonal Antibody, PE-Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61996r-pe-cy3 |
| Product Name | Brachyury Recombinant Monoclonal Antibody, PE-Cy3 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Protein T |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6862 |
| Swiss Prot | O15178 |
| Subcellular location | Nucleus |
| Synonyms | Brachyury homolog; Bry; Protein T; SAVA; TFT; T-box transcription factor T; TBXT; Brachyury protein. |
| Background | Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Brachyury Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61996R-PE-Cy3) validated for, and at what dilution?
bsm-61996R-PE-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Brachyury Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61996R-PE-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61996R-PE-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Brachyury Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61996R-PE-Cy3)?
KLH conjugated synthetic peptide derived from human Protein T
What are the excitation and emission wavelengths of the PE-Cy3 conjugate (bsm-61996R-PE-Cy3)?
PE-Cy3 on bsm-61996R-PE-Cy3: excitation / emission 488nmn/578nm.