PP-1B Recombinant Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61998r-fitc |
| Product Name | PP-1B Recombinant Antibody, FITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PPP1B |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5500 |
| Swiss Prot | P62140 |
| Subcellular location | Nucleus |
| Synonyms | PP1B; PP1beta; PPP1CB; PPP1CD. |
| Background | Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is PP-1B Recombinant Antibody, FITC Conjugated (bsm-61998R-FITC) validated for, and at what dilution?
bsm-61998R-FITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PP-1B Recombinant Antibody, FITC Conjugated (bsm-61998R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61998R-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PP-1B Recombinant Antibody, FITC Conjugated (bsm-61998R-FITC)?
KLH conjugated synthetic peptide derived from human PPP1B
What are the excitation and emission wavelengths of the FITC conjugate (bsm-61998R-FITC)?
FITC on bsm-61998R-FITC: excitation / emission 494nm/518nm.