RNF40 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62002r-cy5 |
| Product Name | RNF40 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RNF40 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9810 |
| Swiss Prot | O75150 |
| Subcellular location | Nucleus |
| Synonyms | BRE 1B; BRE1 B; BRE1B; RBP95; Rnf40; STARING. |
| Background | Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is RNF40 Recombinant Antibody, Cy5 Conjugated (bsm-62002R-Cy5) validated for, and at what dilution?
bsm-62002R-Cy5 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF40 Recombinant Antibody, Cy5 Conjugated (bsm-62002R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62002R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF40 Recombinant Antibody, Cy5 Conjugated (bsm-62002R-Cy5)?
KLH conjugated synthetic peptide derived from human RNF40
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62002R-Cy5)?
Cy5 on bsm-62002R-Cy5: excitation / emission 625,650nm/670nm.