METTL3 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62004r-cy3 |
| Product Name | METTL3 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human METTL3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 56339 |
| Swiss Prot | Q86U44 |
| Subcellular location | Nucleus |
| Synonyms | IME4; M6A; Methyltransferase like protein 3; METTL3; MTA70; N6 adenosine methyltransferase 70 kDa subunit. |
| Background | The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N6 position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is METTL3 Recombinant Antibody, Cy3 Conjugated (bsm-62004R-Cy3) validated for, and at what dilution?
bsm-62004R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does METTL3 Recombinant Antibody, Cy3 Conjugated (bsm-62004R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62004R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise METTL3 Recombinant Antibody, Cy3 Conjugated (bsm-62004R-Cy3)?
KLH conjugated synthetic peptide derived from human METTL3
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62004R-Cy3)?
Cy3 on bsm-62004R-Cy3: excitation / emission 512,550nm/570,615nm.