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HIPK2 Recombinant Antibody, AbBy Fluor-405 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-62009r-bf405
Product Name HIPK2 Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
WB FCM IF(ICC)
Reactivity Human, Mouse
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HIPK2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 28996
Swiss Prot Q9H2X6
Subcellular location Cytoplasm, Nucleus
Synonyms hHIPk2; Hipk2; Nbak1; Stank.
Background Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1, ZBTB4 and DAZAP2.
Application Dilution
WB 1:1000-2000
FCM FCM1μg/Test
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is HIPK2 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62009R-BF405) validated for, and at what dilution?

bsm-62009R-BF405 is validated for WB(WB) (1:1000-2000); FCM(FCM) (1μg/Test); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HIPK2 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62009R-BF405) react with?

Tested reactivity: Human, Mouse.

How should bsm-62009R-BF405 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HIPK2 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62009R-BF405)?

KLH conjugated synthetic peptide derived from human HIPK2

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-62009R-BF405)?

AbBy Fluor-405 on bsm-62009R-BF405: excitation / emission 401nm/421nm.