HIPK2 Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62009r-cy7 |
| Product Name | HIPK2 Recombinant Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HIPK2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 28996 |
| Swiss Prot | Q9H2X6 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | hHIPk2; Hipk2; Nbak1; Stank. |
| Background | Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1, ZBTB4 and DAZAP2. |
| Application Dilution | |
| WB | 1:1000-2000 |
| FCM | FCM1μg/Test |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is HIPK2 Recombinant Antibody, Cy7 Conjugated (bsm-62009R-Cy7) validated for, and at what dilution?
bsm-62009R-Cy7 is validated for WB(WB) (1:1000-2000); FCM(FCM) (1μg/Test); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIPK2 Recombinant Antibody, Cy7 Conjugated (bsm-62009R-Cy7) react with?
Tested reactivity: Human, Mouse.
How should bsm-62009R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIPK2 Recombinant Antibody, Cy7 Conjugated (bsm-62009R-Cy7)?
KLH conjugated synthetic peptide derived from human HIPK2
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-62009R-Cy7)?
Cy7 on bsm-62009R-Cy7: excitation / emission 743nm/767nm.