HIPK2 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62009r-pe-cy3 |
| Product Name | HIPK2 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | PE-Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HIPK2 |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 28996 |
| Swiss Prot | Q9H2X6 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | hHIPk2; Hipk2; Nbak1; Stank. |
| Background | Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1, ZBTB4 and DAZAP2. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is HIPK2 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-62009R-PE-Cy3) validated for, and at what dilution?
bsm-62009R-PE-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIPK2 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-62009R-PE-Cy3) react with?
Tested reactivity: Human, Mouse.
How should bsm-62009R-PE-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIPK2 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-62009R-PE-Cy3)?
KLH conjugated synthetic peptide derived from human HIPK2
What are the excitation and emission wavelengths of the PE-Cy3 conjugate (bsm-62009R-PE-Cy3)?
PE-Cy3 on bsm-62009R-PE-Cy3: excitation / emission 488nmn/578nm.