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NEK2 Recombinant Antibody, Cy5 Conjugated

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bsm-62026r-cy5
Product Name NEK2 Recombinant Antibody, Cy5 Conjugated
Applications
WB
Reactivity Human
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NEK2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4751
Swiss Prot P51955
Subcellular location Cytoplasm, Nucleus
Synonyms HsPK21; NEK2; NEK2A; NLK1.
Background Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is NEK2 Recombinant Antibody, Cy5 Conjugated (bsm-62026R-Cy5) validated for, and at what dilution?

bsm-62026R-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NEK2 Recombinant Antibody, Cy5 Conjugated (bsm-62026R-Cy5) react with?

Tested reactivity: Human.

How should bsm-62026R-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NEK2 Recombinant Antibody, Cy5 Conjugated (bsm-62026R-Cy5)?

KLH conjugated synthetic peptide derived from human NEK2

What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62026R-Cy5)?

Cy5 on bsm-62026R-Cy5: excitation / emission 625,650nm/670nm.