SEC61A1/2 Recombinant Antibody

$The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-SEC61A1\/2 antibody (bsm-62030R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n$

The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-SEC61A1\/2 antibody (bsm-62030R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n

Applications

WB
IHC-P
IHC-F
IP
IF
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62030r
Product Name	SEC61A1/2 Recombinant Antibody
Applications	WB IHC-P IHC-F IP IF FLOW-CYT ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SEC61A1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	29927
Swiss Prot	P61619,Q9H9S3
Subcellular location	Cytoplasm
Synonyms	fb62c11; HSEC61; SEC 61; SEC 61A; Sec61 alpha 1; SEC61; SEC61A1; SEC61A2.
Background	Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.
Application Dilution
WB	1:300-5000
IHC-P	FCM1:20-100
IHC-F	IHC-P1:200-400
IP	IHC-F1:100-500
IF	ICC1:50-200
Flow-Cyt	IP1-2ug
ICC/IF	IF