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AKR1C3 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-62035r-apc-cy5
Product Name AKR1C3 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
WB FCM IF(ICC)
Reactivity Human
Specifications
Conjugation APC-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human AKR1C3
Clonality Monoclonal
Clone # Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8644
Swiss Prot P42330
Subcellular location Cytoplasm
Synonyms DD3; DDX; HA1753; HAKRB; HAKRe; HSD17B5; KIAA0119; hluPGFS.
Background Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(ICC) 1:50-200

Frequently asked questions

Which applications is AKR1C3 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62035R-APC-Cy5) validated for, and at what dilution?

bsm-62035R-APC-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does AKR1C3 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62035R-APC-Cy5) react with?

Tested reactivity: Human.

How should bsm-62035R-APC-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise AKR1C3 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62035R-APC-Cy5)?

KLH conjugated synthetic peptide derived from human AKR1C3

What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-62035R-APC-Cy5)?

APC-Cy5 on bsm-62035R-APC-Cy5: excitation / emission 650nm/670nm.