AKR1C3 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62035r-rbitc |
| Product Name | AKR1C3 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human AKR1C3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8644 |
| Swiss Prot | P42330 |
| Subcellular location | Cytoplasm |
| Synonyms | DD3; DDX; HA1753; HAKRB; HAKRe; HSD17B5; KIAA0119; hluPGFS. |
| Background | Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is AKR1C3 Recombinant Antibody, RBITC Conjugated (bsm-62035R-RBITC) validated for, and at what dilution?
bsm-62035R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AKR1C3 Recombinant Antibody, RBITC Conjugated (bsm-62035R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62035R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AKR1C3 Recombinant Antibody, RBITC Conjugated (bsm-62035R-RBITC)?
KLH conjugated synthetic peptide derived from human AKR1C3
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-62035R-RBITC)?
RBITC on bsm-62035R-RBITC: excitation / emission 570nm/595nm.