EBF1 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62038r-cy3 |
| Product Name | EBF1 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human COE1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1879 |
| Swiss Prot | Q9UH73 |
| Subcellular location | Nucleus |
| Synonyms | COE1; EBF; Ebf1; O/E 1; OE1; OLF1. |
| Background | Key pioneer transcription factor of B-cell specification and commitment.Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is EBF1 Recombinant Antibody, Cy3 Conjugated (bsm-62038R-Cy3) validated for, and at what dilution?
bsm-62038R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EBF1 Recombinant Antibody, Cy3 Conjugated (bsm-62038R-Cy3) react with?
Tested reactivity: Human, Mouse.
How should bsm-62038R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EBF1 Recombinant Antibody, Cy3 Conjugated (bsm-62038R-Cy3)?
KLH conjugated synthetic peptide derived from human COE1
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62038R-Cy3)?
Cy3 on bsm-62038R-Cy3: excitation / emission 512,550nm/570,615nm.