PCYT1A Recombinant Antibody

$4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PCYT1A) monoclonal Antibody, unconjugated (bsm-62059R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PCYT1A) monoclonal Antibody, unconjugated (bsm-62059R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$25 ug total protein per lane of various lysates (see on figure) probed with PCYT1A monoclonal antibody, unconjugated (bsm-62059R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with PCYT1A monoclonal antibody, unconjugated (bsm-62059R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
FCM
IF(ICC)
IP

Reactivity

Human

Overview
Catalog #	bsm-62059r-100ul
Product Name	PCYT1A Recombinant Antibody
Applications	WB, FCM, IF(ICC), IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PCYT1A
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5130
Swiss Prot	P49585
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	CCT A; CCT alph; CTPCT; PCYT1; Pcyt1a.
Background	Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100
IF(ICC)	IFICC1:50-200
IP	IP1:20-50