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TNFSF13 Recombinant Antibody

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-TNFSF13 antibody (bsm-62065R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-TNFSF13 antibody (bsm-62065R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
Bioss unconjugated antibody vial

Applications

  • WB
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62065r
Product Name TNFSF13 Recombinant Antibody
Applications
WB ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TNFSF13
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 8741
Swiss Prot O75888
Subcellular location Secreted
Synonyms APRIL; CD256; TALL2; TNFSF13; TRDL1; ZTNF2.
Background Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
Application Dilution
WB 1:300-5000
ICC/IF IFICC1:50-200

Frequently asked questions

Which applications is TNFSF13 Recombinant Antibody (bsm-62065R) validated for, and at what dilution?

bsm-62065R is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TNFSF13 Recombinant Antibody (bsm-62065R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62065R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise TNFSF13 Recombinant Antibody (bsm-62065R)?

KLH conjugated synthetic peptide derived from human TNFSF13

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