TNNI1 Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI1 Monoclonal Antibody, Unconjugated (bsm-62102R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI1 Monoclonal Antibody, Unconjugated (bsm-62102R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI1 | ssTnI Antibody, Unconjugated (bsm-62102R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI1 | ssTnI Antibody, Unconjugated (bsm-62102R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IHC-P
IHC-F
IP
IF

Reactivity

Human

Overview
Catalog #	bsm-62102r
Product Name	TNNI1 Recombinant Antibody
Applications	WB IHC-P IHC-F IP IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TNNI1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7135
Swiss Prot	P19237
Subcellular location	Cytoplasm
Synonyms	SSTNI; TNN1; Tnni1; Troponin I.
Background	Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Application Dilution
WB	1:500-2000
IHC-P	1:50-100
IHC-F	1:50-200
IP	1-2ug
IF