Zinc Alpha 2 Glycoprotein Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Zinc Alpha 2 Glycoprotein monoclonal antibody, unconjugated (bsm-62121R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Zinc Alpha 2 Glycoprotein monoclonal antibody, unconjugated (bsm-62121R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human white adipose tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Zinc Alpha 2 Glycoprotein Monoclonal Antibody, Unconjugated (bsm-62121R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human white adipose tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Zinc Alpha 2 Glycoprotein Monoclonal Antibody, Unconjugated (bsm-62121R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Zinc Alpha 2 Glycoprotein Monoclonal Antibody, Unconjugated (bsm-62121R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Zinc Alpha 2 Glycoprotein Monoclonal Antibody, Unconjugated (bsm-62121R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Zinc Alpha 2 Glycoprotein Monoclonal Antibody, Unconjugated (bsm-62121R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Zinc Alpha 2 Glycoprotein Monoclonal Antibody, Unconjugated (bsm-62121R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)
IP

Reactivity

Human

Overview
Catalog #	bsm-62121r-100ul
Product Name	Zinc Alpha 2 Glycoprotein Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF(IHC-P), IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human AZGP1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	563
Swiss Prot	P25311
Subcellular location	Secreted
Synonyms	Azgp1; ZA2G; ZAG; ZNGP1.
Background	Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.
Application Dilution
WB	1:500-2000
IHC-P	IHC-P1:100-500
IHC-F	IHC-F1:100-500
IF(IHC-P)	IFIHC-P1:100-500
IP	IP1:20-50