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Peroxiredoxin-5 Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-5 Monoclonal Antibody, Unconjugated (bsm-62124R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-5 Monoclonal Antibody, Unconjugated (bsm-62124R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-5 Monoclonal Antibody, Unconjugated (bsm-62124R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-5 Monoclonal Antibody, Unconjugated (bsm-62124R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-5 Monoclonal Antibody, Unconjugated (bsm-62124R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-5 Monoclonal Antibody, Unconjugated (bsm-62124R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-5 Monoclonal Antibody, Unconjugated (bsm-62124R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-5 Monoclonal Antibody, Unconjugated (bsm-62124R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-5 Monoclonal Antibody, Unconjugated (bsm-62124R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-5 Monoclonal Antibody, Unconjugated (bsm-62124R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IP
  • IF
  • Flow-Cyt

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62124r
Product Name Peroxiredoxin-5 Recombinant Antibody
Applications
WB IHC-P IHC-F IP IF FLOW-CYT
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PRDX5
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified Polyclonal A
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 25824
Swiss Prot P30044
Subcellular location Cytoplasm
Synonyms ACR1; AOEB166; B166; Peroxiredoxin V; Peroxiredoxin-5; PLP; PMP20; PRDX5; PRDX6; Prx-V; PRXV; SBBI10; Thioredoxin reductase.
Background Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IP 1-2ug
IF
Flow-Cyt 1ug/Test

Frequently asked questions

Which applications is Peroxiredoxin-5 Recombinant Antibody (bsm-62124R) validated for, and at what dilution?

bsm-62124R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IP (1-2ug); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Peroxiredoxin-5 Recombinant Antibody (bsm-62124R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62124R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Peroxiredoxin-5 Recombinant Antibody (bsm-62124R)?

KLH conjugated synthetic peptide derived from human PRDX5

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