G3BP1 Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62130r-bf488-100uL |
| Product Name | G3BP1 Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human G3BP1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10146 |
| Swiss Prot | Q13283 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | G3BP; G3bp1; hDH VIII. |
| Background | Protein involved in various processes, such as stress granule formation and innate immunity.Plays an essential role in stress granule formation.Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress.Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations. |
| Application Dilution | |
| WB | 1:1000-2000 |
| FCM | FCM1μg/Test |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is G3BP1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-62130R-BF488) validated for, and at what dilution?
bsm-62130R-BF488 is validated for WB(WB) (1:1000-2000); FCM(FCM) (1μg/Test); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does G3BP1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-62130R-BF488) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62130R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise G3BP1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-62130R-BF488)?
KLH conjugated synthetic peptide derived from human G3BP1
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-62130R-BF488)?
AbBy Fluor-488 on bsm-62130R-BF488: excitation / emission 499nm/519nm.