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RBM8A Recombinant Antibody, AbBy Fluor-647 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62134r-bf647-100uL
Product Name RBM8A Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RBM8A
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9939
Swiss Prot Q9Y5S9
Synonyms BOV1; BOV1A; BOV1B; BOV1C; HSPC114; MDS014; RBM 8; RBM 8A; RBM 8B; RBM8; rbm8a; RBM8B; ZNRP; ZRNP1.
Background Required for pre-mRNA splicing as component of the spliceosome.Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is RBM8A Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-62134R-BF647) validated for, and at what dilution?

bsm-62134R-BF647 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RBM8A Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-62134R-BF647) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62134R-BF647 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RBM8A Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-62134R-BF647)?

KLH conjugated synthetic peptide derived from human RBM8A

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-62134R-BF647)?

AbBy Fluor-647 on bsm-62134R-BF647: excitation / emission 650nm/665nm.