MAG Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with MAG monoclonal antibody, unconjugated (bsm-62144R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with MAG monoclonal antibody, unconjugated (bsm-62144R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62144r
Product Name	MAG Recombinant Antibody
Applications	WB FCM IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MAG
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4099
Swiss Prot	P20916
Subcellular location	Cell membrane
Synonyms	GMA; MAG; S MAG; SIGLEC4A; SPG75.
Background	Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100
IP	IP1:20-50