Pokemon/ZBTB7A Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon\/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon\/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon\/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon\/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon\/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon\/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon\/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon\/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon\/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon\/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with Pokemon\/ZBTB7A monoclonal antibody, unconjugated (bsm-62165R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Pokemon\/ZBTB7A monoclonal antibody, unconjugated (bsm-62165R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Pokemon\/ZBTB7A) monoclonal Antibody, unconjugated (bsm-62165R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Pokemon\/ZBTB7A) monoclonal Antibody, unconjugated (bsm-62165R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$The HT-29 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Pokemon\/ZBTB7A antibody (bsm-62165R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The HT-29 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Pokemon\/ZBTB7A antibody (bsm-62165R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

$4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Pokemon\/ZBTB7A) monoclonal Antibody, unconjugated (bsm-62165R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.$

4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Pokemon\/ZBTB7A) monoclonal Antibody, unconjugated (bsm-62165R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(ICC)
IP

Reactivity

Human

Overview
Catalog #	bsm-62165r
Product Name	Pokemon/ZBTB7A Recombinant Antibody
Applications	WB FCM IHC-P IHC-F IF(IHC-P) IF(ICC) IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ZBTB7A
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51341
Swiss Prot	O95365
Subcellular location	Nucleus
Synonyms	FBI1; LRF; Pokemon; TIP21; ZBTB7; ZBTB7A; ZNF857A.
Background	Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation.
Application Dilution
WB	1:1000-2000
FCM	FCM1:50-100
IHC-P	IHC-P1:200-800
IHC-F	IHC-F1:200-800
IF(IHC-P)	IFIHC-P1:200-800
IF(ICC)	IFICC1:100-500
IP	IP1:20-50