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Galactosidase alpha Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with Galactosidase alpha monoclonal antibody, unconjugated (bsm-62171R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Galactosidase alpha monoclonal antibody, unconjugated (bsm-62171R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galactosidase alpha Monoclonal Antibody, Unconjugated (bsm-62171R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galactosidase alpha Monoclonal Antibody, Unconjugated (bsm-62171R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galactosidase alpha Monoclonal Antibody, Unconjugated (bsm-62171R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galactosidase alpha Monoclonal Antibody, Unconjugated (bsm-62171R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(ICC)
  • IP

Reactivity

  • Human
Overview
Catalog # bsm-62171r
Product Name Galactosidase alpha Recombinant Antibody
Applications
WB FCM IHC-P IHC-F IF(IHC-P) IF(ICC) IP
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human alpha Galactosidase A
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2717
Swiss Prot P06280
Subcellular location Cytoplasm
Synonyms Alpha gal A; GALA; Galactosidase, alpha; GLA; Melibiase.
Background Catalyzes the hydrolysis of glycosphingolipids and participates in their degradation in the lysosome.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IHC-P IHC-P1:50-200
IHC-F IHC-F1:50-200
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200
IP IP1:20-50

Frequently asked questions

Which applications is Galactosidase alpha Recombinant Antibody (bsm-62171R) validated for, and at what dilution?

bsm-62171R is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IHC-P(IHC-P) (1:50-200); IHC-F(IHC-F) (1:50-200); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Galactosidase alpha Recombinant Antibody (bsm-62171R) react with?

Tested reactivity: Human.

How should bsm-62171R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Galactosidase alpha Recombinant Antibody (bsm-62171R)?

KLH conjugated synthetic peptide derived from human alpha Galactosidase A

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