PRKRA Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62172r-rbitc |
| Product Name | PRKRA Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PRKRA |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8575 |
| Swiss Prot | O75569 |
| Subcellular location | Cytoplasm |
| Synonyms | RAX; PACT; DYT16; HSD14. |
| Background | Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-200 |
| IF | |
Frequently asked questions
Which applications is PRKRA Recombinant Antibody, RBITC Conjugated (bsm-62172R-RBITC) validated for, and at what dilution?
bsm-62172R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PRKRA Recombinant Antibody, RBITC Conjugated (bsm-62172R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62172R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PRKRA Recombinant Antibody, RBITC Conjugated (bsm-62172R-RBITC)?
KLH conjugated synthetic peptide derived from human PRKRA
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-62172R-RBITC)?
RBITC on bsm-62172R-RBITC: excitation / emission 570nm/595nm.