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TSC1 Recombinant Antibody, PE-Cy7 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
Overview
Catalog # bsm-62173r-pe-cy7
Product Name TSC1 Recombinant Antibody, PE-Cy7 Conjugated
Applications
WB FCM
Reactivity Human
Specifications
Conjugation PE-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Hamartin
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7248
Swiss Prot Q92574
Subcellular location Cytoplasm, Cell membrane
Synonyms Hamartin; kiaa0243; LAM; TSC; Tsc1; Tuberous sclerosis 1.
Background Non-catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is TSC1 Recombinant Antibody, PE-Cy7 Conjugated (bsm-62173R-PE-Cy7) validated for, and at what dilution?

bsm-62173R-PE-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TSC1 Recombinant Antibody, PE-Cy7 Conjugated (bsm-62173R-PE-Cy7) react with?

Tested reactivity: Human.

How should bsm-62173R-PE-Cy7 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TSC1 Recombinant Antibody, PE-Cy7 Conjugated (bsm-62173R-PE-Cy7)?

KLH conjugated synthetic peptide derived from human Hamartin

What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bsm-62173R-PE-Cy7)?

PE-Cy7 on bsm-62173R-PE-Cy7: excitation / emission 488nm/780nm.