LGR6 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62179r-apc-cy5 |
| Product Name | LGR6 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LGR6 |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified Polyclonal A |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 59352 |
| Swiss Prot | Q9HBX8 |
| Subcellular location | Cell membrane |
| Synonyms | GPCR; Lgr6. |
| Background | Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. May act as a tumor suppressor. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is LGR6 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62179R-APC-Cy5) validated for, and at what dilution?
bsm-62179R-APC-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LGR6 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62179R-APC-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62179R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LGR6 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62179R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human LGR6
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-62179R-APC-Cy5)?
APC-Cy5 on bsm-62179R-APC-Cy5: excitation / emission 650nm/670nm.