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Argininosuccinate synthase 1 Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated(bsm-62194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with Argininosuccinate synthase 1 monoclonal antibody, unconjugated (bsm-62194R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Argininosuccinate synthase 1 monoclonal antibody, unconjugated (bsm-62194R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Argininosuccinate synthase 1 antibody (bsm-62194R,1:100);Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Argininosuccinate synthase 1 antibody (bsm-62194R,1:100);Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IP

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62194r
Product Name Argininosuccinate synthase 1 Recombinant Antibody
Applications
WB FCM IHC-P IHC-F IF(IHC-P) IP
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Argininosuccinate synthase 1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 445
Swiss Prot P00966
Synonyms Argininosuccinate synthase 1; ASS; ass1; ASSA; Citrulline aspartate ligase; CTLN1.
Background One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IHC-P IHC-P1:100-500
IHC-F IHC-F1:100-500
IF(IHC-P) IFIHC-P1:100-500
IP IP1:20-50

Frequently asked questions

Which applications is Argininosuccinate synthase 1 Recombinant Antibody (bsm-62194R) validated for, and at what dilution?

bsm-62194R is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Argininosuccinate synthase 1 Recombinant Antibody (bsm-62194R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62194R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Argininosuccinate synthase 1 Recombinant Antibody (bsm-62194R)?

KLH conjugated synthetic peptide derived from human Argininosuccinate synthase 1

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