Engrailed 1 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-62208r-rbitc |
| Product Name | Engrailed 1 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EN1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2019 |
| Swiss Prot | Q05925 |
| Subcellular location | Nucleus |
| Synonyms | EN1; Engrailed 1; Engrailed; Hu-En-1; Mo-en.1. |
| Background | Belongs to the engrailed homeobox family. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
Frequently asked questions
Which applications is Engrailed 1 Recombinant Antibody, RBITC Conjugated (bsm-62208R-RBITC) validated for, and at what dilution?
bsm-62208R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Engrailed 1 Recombinant Antibody, RBITC Conjugated (bsm-62208R-RBITC) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bsm-62208R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Engrailed 1 Recombinant Antibody, RBITC Conjugated (bsm-62208R-RBITC)?
KLH conjugated synthetic peptide derived from human EN1
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-62208R-RBITC)?
RBITC on bsm-62208R-RBITC: excitation / emission 570nm/595nm.