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HLA-B Recombinant Antibody, AbBy Fluor-647 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bsm-62217r-bf647
Product Name HLA-B Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
WB IF(IHC-P)
Reactivity Human
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HLA B
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3106
Swiss Prot P01889
Subcellular location Cell membrane
Synonyms HLA B; HLAB.
Background Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-B-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells.
Application Dilution
WB 1:500-2000
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is HLA-B Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-62217R-BF647) validated for, and at what dilution?

bsm-62217R-BF647 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HLA-B Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-62217R-BF647) react with?

Tested reactivity: Human.

How should bsm-62217R-BF647 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HLA-B Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-62217R-BF647)?

KLH conjugated synthetic peptide derived from human HLA B

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-62217R-BF647)?

AbBy Fluor-647 on bsm-62217R-BF647: excitation / emission 650nm/665nm.