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RCC1 Recombinant Antibody, Cy5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-62230r-cy5-100uL
Product Name RCC1 Recombinant Antibody, Cy5 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RCC1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1104
Swiss Prot P18754
Subcellular location Cytoplasm, Nucleus
Synonyms RCC1; RCC1-I; SNHG3-RCC1; CHC1.
Background Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is RCC1 Recombinant Antibody, Cy5 Conjugated (bsm-62230R-Cy5) validated for, and at what dilution?

bsm-62230R-Cy5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RCC1 Recombinant Antibody, Cy5 Conjugated (bsm-62230R-Cy5) react with?

Tested reactivity: Human.

How should bsm-62230R-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RCC1 Recombinant Antibody, Cy5 Conjugated (bsm-62230R-Cy5)?

KLH conjugated synthetic peptide derived from human RCC1

What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62230R-Cy5)?

Cy5 on bsm-62230R-Cy5: excitation / emission 625,650nm/670nm.