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KIF4A Recombinant Antibody, Cy7 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-62236r-cy7
Product Name KIF4A Recombinant Antibody, Cy7 Conjugated
Applications
WB FCM IF(ICC)
Reactivity Human, Mouse
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Chromokinesin A
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 24137
Swiss Prot O95239
Subcellular location Cytoplasm, Nucleus
Synonyms Chromokinesin; KIF4; KIF4 G1; KIF4A; KIF4G1.
Background Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis. Translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is KIF4A Recombinant Antibody, Cy7 Conjugated (bsm-62236R-Cy7) validated for, and at what dilution?

bsm-62236R-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does KIF4A Recombinant Antibody, Cy7 Conjugated (bsm-62236R-Cy7) react with?

Tested reactivity: Human, Mouse.

How should bsm-62236R-Cy7 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise KIF4A Recombinant Antibody, Cy7 Conjugated (bsm-62236R-Cy7)?

KLH conjugated synthetic peptide derived from human Chromokinesin A

What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-62236R-Cy7)?

Cy7 on bsm-62236R-Cy7: excitation / emission 743nm/767nm.