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KIF4A Recombinant Antibody, PE-Cy5.5 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-62236r-pe-Cy5.5
Product Name KIF4A Recombinant Antibody, PE-Cy5.5 Conjugated
Applications
WB FCM
Reactivity Human, Mouse
Specifications
Conjugation PE-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Chromokinesin A
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 24137
Swiss Prot O95239
Subcellular location Cytoplasm, Nucleus
Synonyms Chromokinesin; KIF4; KIF4 G1; KIF4A; KIF4G1.
Background Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis. Translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is KIF4A Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-62236R-PE-CY5-5) validated for, and at what dilution?

BSM-62236R-PE-CY5-5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does KIF4A Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-62236R-PE-CY5-5) react with?

Tested reactivity: Human, Mouse.

How should BSM-62236R-PE-CY5-5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise KIF4A Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-62236R-PE-CY5-5)?

KLH conjugated synthetic peptide derived from human Chromokinesin A

What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BSM-62236R-PE-CY5-5)?

PE-Cy5.5 on BSM-62236R-PE-CY5-5: excitation / emission 488nm/694nm.