Gemin 3 Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62248r-cy7-100uL |
| Product Name | Gemin 3 Recombinant Antibody, Cy7 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DDX20 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11218 |
| Swiss Prot | Q9UHI6 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ddx20; DP103; Gemin3; SMN interacting protein. |
| Background | The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is Gemin 3 Recombinant Antibody, Cy7 Conjugated (bsm-62248R-Cy7) validated for, and at what dilution?
bsm-62248R-Cy7 is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Gemin 3 Recombinant Antibody, Cy7 Conjugated (bsm-62248R-Cy7) react with?
Tested reactivity: Human, Mouse.
How should bsm-62248R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Gemin 3 Recombinant Antibody, Cy7 Conjugated (bsm-62248R-Cy7)?
KLH conjugated synthetic peptide derived from human DDX20
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-62248R-Cy7)?
Cy7 on bsm-62248R-Cy7: excitation / emission 743nm/767nm.