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Glutamate dehydrogenase 1 Recombinant Antibody, APC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62249r-apc
Product Name Glutamate dehydrogenase 1 Recombinant Antibody, APC Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Glutamate dehydrogenase 1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2746
Swiss Prot P00367
Synonyms GDH; Gdh-X; GDH1; GLUD; Glud1; Glud1a; Glud1b; Gludl; MRG-2.
Background Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is Glutamate dehydrogenase 1 Recombinant Antibody, APC Conjugated (bsm-62249R-APC) validated for, and at what dilution?

bsm-62249R-APC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Glutamate dehydrogenase 1 Recombinant Antibody, APC Conjugated (bsm-62249R-APC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62249R-APC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Glutamate dehydrogenase 1 Recombinant Antibody, APC Conjugated (bsm-62249R-APC)?

KLH conjugated synthetic peptide derived from human Glutamate dehydrogenase 1

What are the excitation and emission wavelengths of the APC conjugate (bsm-62249R-APC)?

APC on bsm-62249R-APC: excitation / emission 650nm/660nm.