Phospho-GSK3 A/B (Tyr279/Tyr216) Recombinant Antibody

$Western blot analysis of 293 cell lysate treated with AP. Using Phospho-GSK3 A\/B (Tyr279\/Tyr216) (bsm-62258R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of 293 cell lysate treated with AP. Using Phospho-GSK3 A\/B (Tyr279\/Tyr216) (bsm-62258R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IP
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62258r
Product Name	Phospho-GSK3 A/B (Tyr279/Tyr216) Recombinant Antibody
Applications	WB IHC-P IHC-F IP IF ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GSK3 alpha around the phosphorylation site of Y279
Modification Site	Tyr279/Tyr216
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2931
Swiss Prot	P49840
Subcellular location	Cytoplasm
Synonyms	Glycogen synthase kinase-3 alpha; GSK-3 alpha; GSK3A.
Background	GSK3A a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IP	IFICC1:50-200
IF	IP1-2ug
ICC/IF	IF