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LHX3 Recombinant Antibody, PerCP Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62259r-percp-100uL
Product Name LHX3 Recombinant Antibody, PerCP Conjugated
Applications
WB FCM
Reactivity Human, Mouse, Rat
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LHX3
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8022
Swiss Prot Q9UBR4
Subcellular location Nucleus
Synonyms CPHD3; LHX3; Lim3; M2 LHX3; mLim-3; mLIM3; P LIM.
Background Transcription factor. Recognizes and binds to the consensus sequence motif 5'-AATTAATTA-3' in the regulatory elements of target genes, such as glycoprotein hormones alpha chain CGA and visual system homeobox CHX10, positively modulating transcription; transcription can be co-activated by LDB2. Synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is LHX3 Recombinant Antibody, PerCP Conjugated (bsm-62259R-PerCP) validated for, and at what dilution?

bsm-62259R-PerCP is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does LHX3 Recombinant Antibody, PerCP Conjugated (bsm-62259R-PerCP) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62259R-PerCP be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise LHX3 Recombinant Antibody, PerCP Conjugated (bsm-62259R-PerCP)?

KLH conjugated synthetic peptide derived from human LHX3

What are the excitation and emission wavelengths of the PerCP conjugate (bsm-62259R-PerCP)?

PerCP on bsm-62259R-PerCP: excitation / emission 482nm/677nm.