HRH3 Recombinant Antibody, PE-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62260r-pe-cy5-100uL |
| Product Name | HRH3 Recombinant Antibody, PE-Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Histamine H3 receptor |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11255 |
| Swiss Prot | Q9Y5N1 |
| Subcellular location | Cell membrane |
| Synonyms | GPCR97; H3R; HH3R; Histamine H3 receptor; HRH3. |
| Background | The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 neither modified adenylate cyclase activity nor induced intracellular calcium mobilization. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
Frequently asked questions
Which applications is HRH3 Recombinant Antibody, PE-Cy5 Conjugated (bsm-62260R-PE-Cy5) validated for, and at what dilution?
bsm-62260R-PE-Cy5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HRH3 Recombinant Antibody, PE-Cy5 Conjugated (bsm-62260R-PE-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62260R-PE-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HRH3 Recombinant Antibody, PE-Cy5 Conjugated (bsm-62260R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human Histamine H3 receptor
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bsm-62260R-PE-Cy5)?
PE-Cy5 on bsm-62260R-PE-Cy5: excitation / emission 488nm/670nm.