ALOX5AP Recombinant Antibody

$THP-1 (H) cells were treated with or without PMA (80nM) for 72 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with ALOX5AP monoclonal antibody, unconjugated (bsm-62261R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

THP-1 (H) cells were treated with or without PMA (80nM) for 72 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with ALOX5AP monoclonal antibody, unconjugated (bsm-62261R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-62261r
Product Name	ALOX5AP Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human FLAP
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	241
Swiss Prot	P20292
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	ALOX 5AP; ALOX5 AP; ALOX5AP; FLAP.
Background	Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.
Application Dilution
WB	1:500-2000