RYK Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62264r-cy3-100uL |
| Product Name | RYK Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RYK |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6259 |
| Swiss Prot | P34925 |
| Subcellular location | Nucleus |
| Synonyms | ERK 3; JTK5; JTK5A; Ryk; RYK1; Vik. |
| Background | May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is RYK Recombinant Antibody, Cy3 Conjugated (bsm-62264R-Cy3) validated for, and at what dilution?
bsm-62264R-Cy3 is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RYK Recombinant Antibody, Cy3 Conjugated (bsm-62264R-Cy3) react with?
Tested reactivity: Human.
How should bsm-62264R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RYK Recombinant Antibody, Cy3 Conjugated (bsm-62264R-Cy3)?
KLH conjugated synthetic peptide derived from human RYK
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62264R-Cy3)?
Cy3 on bsm-62264R-Cy3: excitation / emission 512,550nm/570,615nm.