PA26 Recombinant Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62280r-bf594-100uL |
| Product Name | PA26 Recombinant Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Sestrin 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27244 |
| Swiss Prot | Q9Y6P5 |
| Subcellular location | Nucleus |
| Synonyms | PA26; sesn1; SEST1; sestrin 1. |
| Background | Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway. |
| Application Dilution | |
| WB | 1:300-5000 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is PA26 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-62280R-BF594) validated for, and at what dilution?
bsm-62280R-BF594 is validated for WB (1:300-5000); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PA26 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-62280R-BF594) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62280R-BF594 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PA26 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-62280R-BF594)?
KLH conjugated synthetic peptide derived from human Sestrin 1
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-62280R-BF594)?
AbBy Fluor-594 on bsm-62280R-BF594: excitation / emission 590nm/617nm.